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For producing single cell protein from the agricultural waste, heat treatment and antibiotics on the growth of Cellulomonas sp. KL-6, isolated in rotting leaf and the adjacent soil mixture, were examined. The organism was able to grow until 5 min. at 65℃, 1 min. at 75℃ and ¼ min. at 85℃ in gradually rising temperatures. It can be Seen that preheating the suspension at 48℃ results in a marked decrease in heat resistance. On heating at temperature of 55℃ for 30 min., strain KL-6 was more resisted in the 0.1 M phosphate buffer when such substrates as casamino acid (1%), yeast extract (1%) or xylose (5%) were added to it whereas this organism was appeared weaker resistances in 0.1 M phosphate buffer when cysteine (0.03 M), sodium citrate (1%) or casein (1%) were in fused into it. Test strain was susceptible to penicillin-G (1.563 ㎍/㎖) and ampicillin (3.125 ㎍/㎖), but the organism was resisted to kanamycin ($gt;200 ㎍/㎖). The treatment of strain KL6 with sodium dodecyl sulfate (SDS) resulted in the elimination of R-plasmid from the host strain and the elimination rate with SDS (10∼30 ㎍/㎖) was about 9.2∼31.2%, respectively.
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